Functional Expression and Characterization of Trichome-Specific (-)-Limonene Synthase and (+)-α-Pinene Synthase from Cannabis sativa
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چکیده
منابع مشابه
Monoterpene Synthases from Grand Fir (Abies grandis) cDNA ISOLATION, CHARACTERIZATION, AND FUNCTIONAL EXPRESSION OF MYRCENE SYNTHASE, (2)- (4S)-LIMONENE SYNTHASE, AND (2)-(1S,5S)-PINENE SYNTHASE*
Grand fir (Abies grandis) has been developed as a model system for studying defensive oleoresin formation in conifers in response to insect attack or other injury. The turpentine fraction of the oleoresin is a complex mixture of monoterpene (C10) olefins in which (2)limonene and (2)-aand (2)-b-pinene are prominent components; (2)-limonene and (2)-pinene synthase activities are also induced upon...
متن کاملMonoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.
Grand fir (Abies grandis) has been developed as a model system for studying defensive oleoresin formation in conifers in response to insect attack or other injury. The turpentine fraction of the oleoresin is a complex mixture of monoterpene (C10) olefins in which (-)-limonene and (-)-alpha- and (-)-beta-pinene are prominent components; (-)-limonene and (-)-pinene synthase activities are also in...
متن کاملMolecular cloning, functional expression and characterization of d-limonene synthase from Agastache rugosa.
We cloned the gene of d-limonene synthase (ArLMS) from Agastache rugosa (Labiatae). The function of ArLMS was elucidated by the preparation of recombinant protein and subsequent enzyme assay. ArLMS consisted of 2077 nucleotides including 1839 bp of coding sequence that encodes a protein of 613 amino acids. This protein has a 60 kDa molecular weight, which is identical to that of d-limonene synt...
متن کاملEvaluation of Gene Expression Level of Limonene and Flavone Synthase and Essential Oil Composition under Different Water Conditions in Cumin
Cumin, Cuminum cyminum L., is the king of spices with a plethora of natural compounds with pharmacological features. Drought stress is a well-known factor that influences the production of some metabolites. We studied the impact of drought stress on gene expression and metabolite content in flower and leaf tissue organs of two ecotypes, Taybad and Ardakan populations. Plants were imposed into t...
متن کاملKinetic characterization, crystallization, and photosynthetic expression of (+)-4R-limonene synthase from C. sinensis
iv TABLE OF CONTENTS v LIST OF TABLES vi LIST OF FIGURES vi
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ژورنال
عنوان ژورنال: Natural Product Communications
سال: 2007
ISSN: 1934-578X,1555-9475
DOI: 10.1177/1934578x0700200301